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This week went by so fast. I had enough homework to keep me occupied for Every. Single. Waking. Moment. this week, so not only am I sleep-deprived, but I also feel as though I haven't relaxed in a year. So much for Vermont.
Oh well, the good news is that it was a GOOD week, even if it didn't let up and seemed to soar by. I really do enjoy my classes, even though I had to read eight papers in three days for them, and I really do love my new lab, even though all I'm doing is cloning and the postdoc in charge of me keeps telling me to do it different ways. I tried to do a DNA extraction this morning using phenol chloroform (super nasty stuff) instead of just using the QIAgen kit, and blah. I'd much rather use the kit. Turns out it really really does matter if you add .6 volumes of isopropanol versus .1 volume, because apparently I can't do math, and no amount of spinning was going to get that DNA to precipitate. I stopped, did a calculation, added the rest of the isopropanol, and was going to spin the damn thing again when I realized someone was using the centrifuge. So I spun it at 4*C, which was okay, 'cause it did precipitate, but I think it's probably better to do it at room temp. I threw the whole thing in the fridge when I was done (well, I washed and resuspended and stuff), and I think I'll just redo it on Monday. I was only in lab for about four hours today before I decided that I just wasn't going to get anything productive done. I left at about 2:00.
I stopped by the med campus (I know, SO not on my way home) to visit the Baserga lab and say hi to Erica. It's getting more and more tempting to join that lab. While I was hanging out today, I looked around and realized that I really miss it. THEY aren't psycho about their gels. (My new lab melts down their gels--yes, WITH the ethidium bromide in them--and pours them again and again and again, until the background gets too high to visualize bands anymore. Isn't that sick? And... really bad for your germ line. I mean, man, I bet that every single person in that lab is going to have mutant babies some day. If I join that lab, I'm gonna totally revamp the gel system.)
Anyway. It was a good visit, and I came home around 3:30 and smashed into bed. I slept for, um, four and a half hours. And I only woke up because of the marching band outside my building (WHY do I live next to the gym???), thinking to myself, "God DAMN it, why is there a marching band outside this frickin' early in the morning?!? It's not even light yet!" And then I realized that it wasn't morning, it was night, and that I had to get up and take a shower so I can go to a party at GPSCY tonight. It's the second recruitment weekend, and there's another GPSCY party, and I really really hope that this weekend doesn't end up as weird as the last one. A repeat of that Friday night would be okay, though.
I wish I had something more interesting to post about. I feel like my life is fairly low-stress right now, which seems to actually make for uninteresting entries. Weird how that works out. Not that I'm complaining about life being low-stress, but it WOULD be nice to have motivation to write interesting things. Maybe I should start writing fiction.
Oh well, the good news is that it was a GOOD week, even if it didn't let up and seemed to soar by. I really do enjoy my classes, even though I had to read eight papers in three days for them, and I really do love my new lab, even though all I'm doing is cloning and the postdoc in charge of me keeps telling me to do it different ways. I tried to do a DNA extraction this morning using phenol chloroform (super nasty stuff) instead of just using the QIAgen kit, and blah. I'd much rather use the kit. Turns out it really really does matter if you add .6 volumes of isopropanol versus .1 volume, because apparently I can't do math, and no amount of spinning was going to get that DNA to precipitate. I stopped, did a calculation, added the rest of the isopropanol, and was going to spin the damn thing again when I realized someone was using the centrifuge. So I spun it at 4*C, which was okay, 'cause it did precipitate, but I think it's probably better to do it at room temp. I threw the whole thing in the fridge when I was done (well, I washed and resuspended and stuff), and I think I'll just redo it on Monday. I was only in lab for about four hours today before I decided that I just wasn't going to get anything productive done. I left at about 2:00.
I stopped by the med campus (I know, SO not on my way home) to visit the Baserga lab and say hi to Erica. It's getting more and more tempting to join that lab. While I was hanging out today, I looked around and realized that I really miss it. THEY aren't psycho about their gels. (My new lab melts down their gels--yes, WITH the ethidium bromide in them--and pours them again and again and again, until the background gets too high to visualize bands anymore. Isn't that sick? And... really bad for your germ line. I mean, man, I bet that every single person in that lab is going to have mutant babies some day. If I join that lab, I'm gonna totally revamp the gel system.)
Anyway. It was a good visit, and I came home around 3:30 and smashed into bed. I slept for, um, four and a half hours. And I only woke up because of the marching band outside my building (WHY do I live next to the gym???), thinking to myself, "God DAMN it, why is there a marching band outside this frickin' early in the morning?!? It's not even light yet!" And then I realized that it wasn't morning, it was night, and that I had to get up and take a shower so I can go to a party at GPSCY tonight. It's the second recruitment weekend, and there's another GPSCY party, and I really really hope that this weekend doesn't end up as weird as the last one. A repeat of that Friday night would be okay, though.
I wish I had something more interesting to post about. I feel like my life is fairly low-stress right now, which seems to actually make for uninteresting entries. Weird how that works out. Not that I'm complaining about life being low-stress, but it WOULD be nice to have motivation to write interesting things. Maybe I should start writing fiction.
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Date: 2007-02-17 02:28 am (UTC)From:Glad to hear that life is low stress. That's good sometimes :)
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Date: 2007-02-17 07:36 am (UTC)From: